解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::To elucidate the regulatory pathway through which pancreastatin inhibits insulin secretion, RINm5F insulinoma cells were challenged with physiological and pharmacological probes known to stimulate insulin release through different mechanisms. Utilizing the electrophysiological technique of capacitance measurements as ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(96)90081-0
更新日期:1996-02-01 00:00:00
abstract::Previously we demonstrated that stimulation of cultured neonatal rat ventricular myocytes by either alpha 1-adrenergic agonist or endothelin-1 resulted in a rapid formation of total inositolphosphates, although the levels of inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate did not rise significantly...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(95)90014-4
更新日期:1995-12-01 00:00:00
abstract::A new method for the determination of tissue concentrations of Fura-2 and Fura-2/AM was developed based upon acetonitrile extraction followed by RP-HPLC separation (using tetrahexylammonium as counter-ion), post-column alkaline hydrolysis of Fura-2/AM, and fluorimetric detection. The detection limit was 1.2 nM and 1 n...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(95)90057-8
更新日期:1995-11-01 00:00:00
abstract::A decrease of cytoplasmic Ca(2+)-concentration in vertebrate photoreceptor cells after illumination is necessary for light adaptation. Although the mechanisms of adaptation is not completely understood, several Ca(2+)-dependent cellular processes have been discovered. Some involve calcium-binding proteins like recover...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/0143-4160(95)90027-6
更新日期:1995-10-01 00:00:00
abstract::We previously found in single channel studies that ryanodine receptor (RyR) channel activity can be made insensitive to block by Mg2+ when terminal cisternae of sarcoplasmic reticulum, incorporated into planar bilayers, are treated with protein kinase A (PKA) or Ca2+/calmodulin dependent protein kinase type II (CamPK ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(95)90064-0
更新日期:1995-09-01 00:00:00
abstract::The effect of furosemide and ouabain on the intracellular concentration of Ca2+ was studies in myocardial cell cultures using Fura-2, a fluorescent agent, as an intracellular Ca2+ indicator. Introduction of 200 microM ouabain to the cultured cells increased the intracellular calcium concentration from an average of 23...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(95)90004-7
更新日期:1995-08-01 00:00:00
abstract::An inward current responsible for hormone regulated Ca2+ entry has been identified in cultured rat hepatocytes using whole cell patch clamp. Addition of 20 nM vasopressin or of 100 microM ATP induced the inward current, which could be observed more clearly after blocking an outward K+ current. This large outward K+ cu...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(95)90042-x
更新日期:1995-07-01 00:00:00
abstract::Adipokinetic hormone (AKH)-induced mobilization of insect fat body glycogen occurs through activation of glycogen phosphorylase. In the migratory locust, signal transduction of AKH-I, -II and -III has been shown to involve the formation of cAMP. In the present study, we show that both the elevation of fat body cAMP le...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(95)90092-6
更新日期:1995-06-01 00:00:00
abstract::The mechanisms that regulate ion and fluid transport by the human intrahepatic bile duct have not been well defined. Human intrahepatic biliary cell lines that we have developed were used to identify and characterize purinoceptors based on increases in intracellular calcium in response to ATP and other nucleotides. In...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(95)90111-6
更新日期:1995-05-01 00:00:00
abstract::A possible role of the nitric oxide (NO)/cGMP pathway in the regulation of Ca2+ entry into HT29/B6 human colonic epithelial cells was investigated using digital image processing of Fura-2 fluorescence and immunoblotting for nitric oxide synthase (NOS). We tested the hypothesis that Ca2+ store depletion causes increase...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(95)90071-3
更新日期:1995-04-01 00:00:00
abstract::The ability of the ectodermal cells to be induced and to differentiate toward neural tissue, called neural competence, is acquired shortly before gastrulation and lost during late gastrula stages in Pleurodeles waltl embryos. We have examined ectodermal cells' neural competence in relation to the evolution of the dens...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(95)90036-5
更新日期:1995-03-01 00:00:00
abstract::We analyzed spatio-temporal characteristics of Ca2+ transients in the cytosol and the nucleus of cultured neonatal rat heart cells using confocal imaging with Indo-1 and Fluo-3. In resting heart muscle cells, nuclear [Ca2+] was maintained lower than the cytosolic level. The rise in nuclear [Ca2+], during either E-C co...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(95)90031-4
更新日期:1995-03-01 00:00:00
abstract::Extracellular nucleotides are potent Ca2+ mobilizing agents. A variety of receptors for extracellular ATP are recognised. Some are involved in fast neuronal transmission and operate as ligand-gated ion channels. Others are involved in the paracrine or autocrine modulation of cell function. Many receptors of this type ...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/0143-4160(95)90080-2
更新日期:1995-02-01 00:00:00
abstract::Previously, we have used the classical approach to examine intracellular calcium stores in human foreskin fibroblasts (HSWP) cells. In this classical protocol cells are first permeabilized and then allowed to fill their Ca2+ reservoirs with 45Ca2+ in the presence of ATP. In this paper we present an alternative method ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(95)90101-9
更新日期:1995-01-01 00:00:00
abstract::Signaling between intracellular Ca2+ stores and cell membrane channels or transporters is important to Ca(2+)-based second messenger systems. Two hypotheses, the capacitative and the Ca(2+)-induced Ca(2+)-influx models have been proposed to explain aspects of this signaling. In this study, we examined the applicabilit...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(94)90076-0
更新日期:1994-12-01 00:00:00
abstract::We have examined the internal Ca(2+)-dependence of the long-term evolution of whole cell high voltage activated Ca current in chromaffin cells. The evolution of the peak Ca current was characterized by 2 distinct phases: after an initial facilitation, there followed a rundown, which represented a reduction by 70% with...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(94)90029-9
更新日期:1994-11-01 00:00:00
abstract::IP3R is located to the inner nuclear membrane. Nuclear IP3R is recognized as a 220 kD immunoreactive protein by antisera raised against purified rat brain IP3R. Antisera against C-terminal 95-108 peptide fragment derived from rat brain IP3R does not reveal immunoreactivity in the nucleus. Nuclear IP3R is sensitive to ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(94)90094-9
更新日期:1994-10-01 00:00:00
abstract::In the presence of inositol (1,4,5)-trisphosphate (InsP3) repetitive waves of elevated cytosolic free Ca2+ (Ca waves) that travel through cellular cytoplasm are observed. Investigation of this phenomenon stimulated the view of cellular cytoplasm as 'an excitable medium composed of Ca release processes (InsP3R), couple...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(94)90019-1
更新日期:1994-09-01 00:00:00
abstract::An initial model has been proposed to describe a mechanism for cytosolic calcium oscillations [Jafri MS. Vajda S. Pasik P. Gillo B. (1992) A membrane model for cytosolic calcium oscillations: a study using Xenopus oocytes. Biophys. J., 63, 235-246]. In this paper we extend our original model to include the effects of ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(05)80003-x
更新日期:1994-07-01 00:00:00
abstract::Mathematical models are often used to elucidate mechanisms behind cytosolic Ca2+ oscillations. We have evaluated the use of mathematical modelling to analyse and quantify Ca2+ signal patterns, in single, adherent human neutrophils (PMN) after stimulation by the bacterial peptide N-formyl-methionyl-leucyl-phenylalanine...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(94)90111-2
更新日期:1994-06-01 00:00:00
abstract::Photolysis of caged calcium (Nitr5, Calbiochem) can be used to study calcium dependent processes such as excitation-contraction coupling and muscular mechanics. Expensive high energy light sources are routinely used for UV light exposure, but this study describes an alternative low cost xenon flash unit constructed in...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(94)90017-5
更新日期:1994-05-01 00:00:00
abstract::Apparent calcium stability constants and the purity of Ca-chelating agents were evaluated using Ca(2+)-selective electrodes by the double-log optimization method [Oiki S. Yamamoto T. Okada Y. (1994) Cell Calcium, 15, 199-208]. The method was amended to allow evaluation of the free Ca2+ concentrations contaminating ele...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(94)90060-4
更新日期:1994-03-01 00:00:00
abstract::The calcium release channel/ryanodine receptor of rabbit skeletal muscle sarcoplasmic reticulum is tightly associated with the immunophilin FK-506 binding protein (FKBP-12). The immunosuppressant drug FK-506 effectively dissociates FKBP-12 from the calcium release channel of terminal cisternae (TC) vesicles. Furthermo...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(94)90048-5
更新日期:1994-02-01 00:00:00
abstract::The effect of palmitoyl-CoA (PCoA) on Ca2+ fluxes in unfractionated SR, longitudinal tubules (LSR) and terminal cisternae (TC) subfractions, obtained from rabbit fast-twitch skeletal muscles, was investigated. After MgATP-dependent Ca2+ preloading, PCoA released Ca2+ from unfractionated SR and TC, but not from LSR. Bo...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(94)90049-3
更新日期:1994-02-01 00:00:00
abstract::The best known calcium waves move at about 5-30 microns/s (at 20 degrees C) and will be called fast waves to distinguish them from slow (contractile) ones which move at 0.1-1 microns/s as well as electrically propagated, ultrafast ones. Fast waves move deep within cells and seem to underlie most calcium signals. Their...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/0143-4160(93)90099-r
更新日期:1993-11-01 00:00:00
abstract::The oscillations in cytosolic Ca2+ evoked in pancreatic exocrine acinar cells by submaximal concentrations of the two phosphoinositidase-coupled agonists acetylcholine (ACh) and cholecystokinin octapeptide (CCK-8) have very different temporal patterns. In the present study we use digital video imaging of Fura-2 fluore...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(93)90096-o
更新日期:1993-11-01 00:00:00
abstract::Muscarinic acetylcholine (ACh) receptors activate the phospholipase C signal transduction pathway to promote the formation of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] and the consequent elevation of cytoplasmic calcium (Ca2+). The inositol phosphate and Ca(2+)-mobilization responses to ACh were analyzed in Xenopus ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(93)90090-s
更新日期:1993-10-01 00:00:00
abstract::Depletion of calcium in the extracellular medium used to incubate first trimester human placental minces resulted in a significant decrease in the quantity of immunoreactive hCG in the medium and a corresponding increase in the tissue. In contrast, when secretion of newly synthesised hCG was monitored in the absence o...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(93)90060-j
更新日期:1993-09-01 00:00:00
abstract::In salivary acinar cells, muscarinic-induced fluid secretion is associated with a 1,4,5-IP3 induced increase in the cytosolic free Ca2+ concentration ([Ca2+]i), which in turn activates Ca(2+)-dependent K+ and Cl- channels that modulate the membrane potential. In the present study the influence of the membrane potentia...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(93)90076-i
更新日期:1993-07-01 00:00:00
abstract::Rat liver was homogenized and subjected to differential centrifugation. When the low speed nuclear pellet was processed on a Percoll gradient, plasma membrane markers and Ins(1,4,5)P3 binding activity purified together. The high speed (microsomal) fraction was subfractionated by sucrose density gradient centrifugation...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(93)90007-s
更新日期:1993-06-01 00:00:00
abstract::Fluorescent calcium indicators have been widely used to assess cytoplasmic calcium concentration in cells. To examine the role of calcium ions on different physiological functions (e.g. in case of liver; bile secretion, glucose metabolism, etc.) there is a need for whole organ studies. We have developed a technique to...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(93)90005-q
更新日期:1993-06-01 00:00:00
abstract::We present a new method for ratiometric Ca2+ measurements using indicators with excitation spectra in the visible range of wavelengths. Laser-scanning confocal microscopy was used to record intracellular Ca(2+)-signals with high temporal and spatial resolution in single cardiac myocytes. The patch-clamp technique was ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(93)90040-d
更新日期:1993-05-01 00:00:00
abstract::The mechanism by which the depletion of intracellular Ca2+ stores stimulates Ca2+ influx is poorly understood. However, the coupling of depletion to influx is broken during mitosis [Preston, S.F. et. al., (1991) Cell Regul., 2, 915-925]. Thus, in interphase HeLa cells, activation of the histamine H1 receptor, or incub...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(93)90042-5
更新日期:1993-05-01 00:00:00
abstract::Calbindin D28K (formerly known as vitamin D-dependent calcium binding protein and referred to here as calbindin) is found in a wide variety of tissues, but only in certain cells within those tissues. Apart from its ability to bind calcium, nothing is known about its function in these cells. To investigate its role we ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(93)90055-b
更新日期:1993-04-01 00:00:00
abstract::The activation of T lymphocytes, like many cells, requires a rapid rise in intracellular Ca2+ secondary to both an influx and a release from intracellular stores. The latter is activated by inositol-1,4,5-trisphosphate [Ins(1,4,5)P3]. It is controversial if inositol phosphates can also stimulate a plasma membrane Ca2+...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(93)90082-h
更新日期:1993-02-01 00:00:00
abstract::Quartered pituitaries from castrated (72 h) +/- estradiol (E2)-treated (24 h) rats were used in a perifusion system to investigate estradiol modulation of ionomycin and ionomycin + PMA stimulated LH secretion. Estradiol enhanced the LH responses to GnRH (1 nM) and ionomycin (10 microM), and was necessary for the manif...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(93)90015-x
更新日期:1993-01-01 00:00:00
abstract::A 106 kD protein was isolated from skeletal sarcoplasmic reticulum (SR) vesicles and shown to have the properties of SR Ca2+ release channels, including blockade by 5 nM ryanodine. In view of extensive reports that the ryanodine-receptor complex consists of four 565 kD junctional feet proteins (JFPs) and is the 'physi...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(92)90074-3
更新日期:1992-11-01 00:00:00
abstract::It is well known that in different tissues, dihydropyridines bind at nanomolar concentrations to a receptor and block voltage-operated Ca2+ channels. In studies reported here, Harderian gland tissue homogenates from intact male hamsters exhibited significant dihydropyridine binding (Bmax = 1700 fmoles/mg protein) of h...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(92)90036-r
更新日期:1992-10-01 00:00:00
abstract::Mechanically induced channel activities and increase of intracellular calcium ([Ca2+]i) in normal and cancerous murine mammary cells (MMT 060562) were investigated using the patch clamp technique and Fura-2 fluorescence. Both cell types showed similar properties. Upon mechanical stimulation, activation of the Ca(2+)-d...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(92)90018-n
更新日期:1992-08-01 00:00:00
abstract::Aluminium lactate provokes the same modification of 45Ca(2+)-uptake by Ehrlich ascites tumour cells as does ferric lactate. The increase of uptake is metal-complex concentration-dependent, and in contrast to ferric lactate, is only partially inhibited by albumin. Under the same experimental conditions, chromic lactate...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(92)90062-w
更新日期:1992-05-01 00:00:00